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Image Search Results
Journal: bioRxiv
Article Title: Rab11FIP2 controls NLRP3 inflammasome activation through Rab11b
doi: 10.1101/2025.05.19.654879
Figure Lengend Snippet: FIP2 locates to the dTGN structures and controls their number ( A ) Confocal images showing NLRP3 in TGN46 positive dTGN structures in primary human macrophages. ( B ) Confocal images showing NLRP3 in TGN46 positive dTGN structures human primary macrophages. The macrophages were LPS primed and treated with nigericin for 2 h (A and B). ( C ) Confocal images showing Flag-FIP2 in microdomains on Rab5 coated endosomes. ( D ) Confocal images showing NLRP3 microdomains co-localizing with FIP2 on a FIP2 coated enlarged endosome. ( E ) Quantification of dTGN numbers and corresponding NLRP3 levels in THP-1 cells stimulated as indicated. 96-126 cells were monitored per condition, in n = 4 independent experiments. ( F ) Quantification of dTGN numbers and corresponding NLRP3 levels in THP-1 cells stimulated as indicated. 62-132 cells were monitored per condition, in n = 1 independent experiment. THP-1 cells were unstimulated or LPS primed for 2 h before 1 h of nigericin treatment. TGN46 positive dTGN structures were identified by the IMARIS 8.2 imaging software and the NLRP3 intensities given as mean voxel intensity. Data information: In (E) data are presented as mean +/-SEM, and (F) as mean +/-SD. **** p < 0.0001 (Two-way ANOVA, Tukey’s multiple comparisons test with adj. p values). White arrows point at dTGN puncta positive for NLRP3 or FIP2; or Rab5 or FIP2 coated endosomes positive for FIP2 or NLRP3, respectively. N = nigericin. Scale bar = 5 μm.
Article Snippet: Rabbit anti-Gasdermin D (#96458), rabbit anti-Gasdermin D (#97558), rabbit anti-phospho-TAK1 (#4508), rabbit anti-phospho-IKKα/IKKβ (16A6) (#2697), rabbit anti-IKKα (#2682) and rabbit anti-IKKβ (D30C6) (#8943),
Techniques: Imaging, Software
Journal: bioRxiv
Article Title: Rab11FIP2 controls NLRP3 inflammasome activation through Rab11b
doi: 10.1101/2025.05.19.654879
Figure Lengend Snippet: ( A ) Confocal image showing PI4P in the TGN-ring of resting cells. ( B ) Confocal image showing that PI4P is depleted form the TGN46 positive ring and appear on puncta endosomal structures following LPS priming. ( C ) Confocal image showing NLRP3 (green) on an enlarged PI4P positive endosome (red). ( D ) Confocal image showing PI4P (green) on an enlarged Rab5 coated endosome (red). ( E ) Quantification of PI4P levels in the TGN before and after LPS priming for 2 h. ( F ) Quantification of PI4P positive endosomes before and after nigericin treatment of LPS primed cells. ( G ) Quantification of PI4P positive endosomes before and after nigericin treatment of LPS primed cells. The cells were primed with 100 ng/mL LPS for 2 h and treated with 5 μM Nigericin. MCC950 were added 30 min prior LPS priming. In (E) data are presented a as mean +/-SEM. **** p<0.0001 (Two-way ANOVA Tukeýs multiple comparisons test with adj. p values) and (F) as mean +/-SD. * p=0.029 (Multiple unpaired t test). Scale bar = 5 μm.
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